EDTA Buffer

2x CTAB buffer (1L) pH 8:

100 mM Tris (pH 8) = 12.11 g

20 mM EDTA (pH 8) = 5.84 g

1.4 M NaCL = 81.8 g

2% CTAB = 20 g

Dissolve by heating 60 C.

Bring the pH to 8 by adding HCL or NaOH.

Autoclave and store at RT.