TRIS 6,057 g

EDS 0,730 g

D-Sorbit 31,885 g

add H2O (L-Chromatography) to bring the total volume to 500 mL 

Let it dissolve on the stirring mixer for about 1 hour

Measure the pH and bring it to 8.0, if needed. If higher, add HCL, if lower, add NaOH

Autoclave with the liquid program and with the lid unscrewed.